Catalog number:  EHRNTS

 Related applications: Protein Assays and Analysis

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Use the Thermo Scientific® Human RANTES ELISA Kit to assay RANTES (regulated upon activation, normal T-cell expressed and secreted) protein levels in human serum, plasma, tissue extracts, urine, and cell culture supernatant samples. This is an alternate product for Cat. Nos. KHC1031 and KAC1511.

This kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of RANTES protein levels in samples. The Thermo Scientific® Human RANTES ELISA Kit also includes calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution, and stop solution. This complete kit contains one pre-coated 96-well strip plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: natural and recombinant human RANTES (regulated upon activation, normal T-cell expressed and secreted) protein
• Assay range: 51.2 to 2000 pg/mL
• Sensitivity: <2 pg/mL
• Sample types: cell culture supernatant, urine, serum, plasma (with EDTA, sodium citrate, heparin)
• Total assay time: 3 hours
• Sample size: 50 μL
• Specificity: no significant cross-reaction with human Eotaxin, GROα, GROβ, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNFα or TNFβ, or rat RANTES
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Chemokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP
Gene ID: 6352
Gene Name: C-C motif chemokine ligand 5
Gene Symbol: CCL5
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: C-C motif chemokine 5
Target (Kit Named): RANTES
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P13501

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols