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<i>E. coli</i> DNA Ligase
Thermo Scientific™

E. coli DNA Ligase

Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.
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Catalog NumberQuantity
EL00361400 U
Catalog number EL00361
Price (USD)
142.00
Each
Quantity:
400 U
Price (USD)
142.00
Each

Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA. The enzyme is not active on blunt-ended DNA.

Features

  • Activity in wide temperature range (4°C to 37°C)
  • High specificity to cohesive DNA ends

Applications

  • Nick ligation in double-stranded DNA
  • cDNA synthesis
  • Cloning

For Research Use Only. Not for use in diagnostic procedures.

Specifications
For Use With (Application)Nick ligation in double-stranded DNA, cDNA synthesis, Cloning, DNA ligation
Inactivation Temperature65°C
Product TypeNAD-dependent DNA Ligase
Quantity400 U
Concentration8 U/μL
EnzymeDNA Ligase
FormLiquid
Research CategoryTraditional Cloning
Sensitive to Heat InactivationYes
Unit SizeEach
Contents & Storage
8 U/μL E.Coli DNA Ligase, 50 μL

Store at -20°C.