Thermo Scientific™

DNase I, RNase-free, HC (50 U/µL)

Catalog number:  EN0523

Related applications:

In Vitro Transcription

| Reverse Transcription

| Real Time PCR (qPCR)

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Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.

In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion. In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides.


• Recombinant enzyme
• Purified from non-animal host with a lower level of intrinsic RNases


• Preparation of DNA-free RNA
• Removal of template DNA following in vitro transcription
• Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
• DNA labeling by nick-translation in conjunction with DNA Polymerase I
• Studies of DNA-protein interactions by DNase I, RNase-free footprinting
• Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used


DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.
For Research Use Only. Not for use in diagnostic procedures.


Concentration: 50U/μL
Quantity: 1000U
Enzyme: DNase