Thermo Scientific Lambda Exonuclease is a highly processive 5'→3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA.
• Active in Thermo Scientific PCR buffers
Applications • Generating single-stranded PCR products for use in: • DNA sequencing • Analysis of DNA single-strand conformation polymorphism (SSCP) • Rolling circle amplification
• Producing single-stranded DNA from double-stranded DNA fragments • Cloning of PCR products
Use of this enzyme in certain applications may be covered by patents and may require a license.
For Research Use Only. Not for use in diagnostic procedures.