T4 beta-glucosyltransferase

Catalog number: EO0831

Thermo Scientific™  Related applications: Methylation Analysis

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Thermo Scientific T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating ß-glucosyl-5 hydroxymethylcytosine.

Thermo Scientific T4 BGT is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes 5-hmC glucosylation of 1 µg DNA at 37°C in 15 minutes.


Specific—selectively transfers glucose to the hydroxymethyl moiety of 5-hmC
Fast—complete glucosylation of 1 µg DNA in just 15 min
Convenient—supplied with optimized buffer and UDP-glucose.


• Locus specific detection of 5-hmC
• Enrichment of 5-hmC containing DNA
• Labeling of 5-hmC residues using a radioactive UDP-glucose donor

• T4 β-glucosyltransferase, 5 U/µL
• 10X Epi buffer
• 10X UDP-glucose
For Research Use Only. Not for use in diagnostic procedures.


Enzyme: T4 β-glucosyltransferase
Product Size: 500 units


Manuals & protocols

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