Thermo Scientific T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating ß-glucosyl-5 hydroxymethylcytosine.
Thermo Scientific T4 BGT is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes 5-hmC glucosylation of 1 µg DNA at 37°C in 15 minutes.
• Specific—selectively transfers glucose to the hydroxymethyl moiety of 5-hmC • Fast—complete glucosylation of 1 µg DNA in just 15 min • Convenient—supplied with optimized buffer and UDP-glucose.
• Locus specific detection of 5-hmC • Enrichment of 5-hmC containing DNA • Labeling of 5-hmC residues using a radioactive UDP-glucose donor