Thermo Scientific™

T7 DNA Polymerase (10 U/µL)

Catalog number: EP0081
Thermo Scientific™

T7 DNA Polymerase (10 U/µL)

Catalog number: EP0081
Catalog Number
EP0081
Unit Size
300 units
Price (USD)
Price: 96.25
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP0081300 units
Price: 96.25
Your Price:
***
Product Overview
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Thermo Scientific T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5'→3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a high 3'→5' exonuclease activity towards single- and double-stranded DNA.

Highlights

Strong 3'→5' exonuclease activity, approximately 1000-fold greater than Klenow Fragment
Active in restriction enzyme buffers

Applications

• Purification of covalently closed circular DNA by removal of residual genomic DNA
• Primer extension reactions on long templates (see​ Reference 1)
• DNA 3'-end labeling (see​ Reference 1)
• Strand extensions in site-directed mutagenesis
• Fill-in blunting of 5'-overhang DNA
• Second strand synthesis of cDNA
In situ detection of DNA fragmentation associated with apoptosis

Note

Assays at 37°C require only short incubation times .
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase
T7 DNA Polymerase
Quantity
300 unit

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