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Thermo Scientific™

phi29 DNA Polymerase (10 U/μL)

Catalog number: EP0091
Thermo Scientific™

phi29 DNA Polymerase (10 U/μL)

Catalog number: EP0091
Catalog Number
EP0091
Unit Size
250 units
Price (USD)
Price: 90.75
Online price: 70.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
1,000 units
Price (USD)
Price: 308.00
Online price: 240.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
5,000 units
Price (USD)
Price: 1,178.00
Online price: 918.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP0091250 units
Price: 90.75
Online price: 70.65
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EP00921,000 units
Price: 308.00
Online price: 240.65
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EP00945,000 units
Price: 1,178.00
Online price: 918.65
Your price:
-
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Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. Produced from E. coli cells with a cloned gene 2 of Bacillus subtilis phage phi29, phi29 DNA Polymerase possesses 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA.

Features of phi29 DNA Polymerase
• Excellent processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized
• Highly accurate DNA synthesis
• High yields of amplified DNA even from small amounts of template

Applications
Phi29 DNA Polymerase may be used in a wide variety of applications including:
• Rolling circle amplification (RCA)—generation of periodic DNA nanotemplates
• Multiple displacement amplification (MDA)
• Unbiased amplification of whole genome (WGA) (see figure below)
• Amplification of DNA for SNP and STR detection
• Cell-free amplification of DNA from single cells, pathogenic organisms, or metagenomes
• DNA template preparation for sequencing
• Protein-primed DNA amplification
• Recombination based-cloning
• Cell-free cloning of lethal DNA
• RNA-primed DNA amplification

Notes
1. The addition of pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis.
2. Because of the enzymes’s 3’→5’ proofreading exonuclease, 3'-modified primers are highly recommended.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Stand-alone enzyme
For Use With (Application)
MDA-WGA, RCA
Polymerase
phi29 DNA Polymerase
Quantity
250 U
Optimal Reaction Temperature
30°C
Shipping Condition
Dry Ice
Concentration
10 U/μL
Reaction Time
4 hours

Contents & Storage

• phi29 DNA Polymerase (250 units at 10 U/μL)
• 10X Reaction Buffer for phi29 DNA Polymerase
• Store at –15 to –25°C.

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