Thermo Scientific™

phi29 DNA Polymerase (10 U/µL)

Catalog number:  EP0091

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses a 3'→5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA or RNA. Therefore 3'-modified primers are highly recommended.


• Highest processivity and strand displacement activity among known DNA polymerases – more than 70 kb long DNA stretches can be synthesized
• Highly accurate DNA synthesis
• Extremely high yields of amplified DNA even from minute amounts of template
• Amplification products can be directly used in downstream applications (PCR, restriction digestion, SNP genotyping, etc.)


• Rolling circle amplification (RCA): generation of periodic DNA nanotemplates
• Multiple displacement amplification (MDA)
• Unbiased amplification of whole genome (WGA, see Figure 1 in Supporting Data):
• amplification of DNA for SNP and STR detection
• cell-free amplification of DNA from single cells
• pathogenic organisms or metagenomes
• amplification of DNA from filter paper blood spot samples
• DNA template preparation for sequencing
• Protein-primed DNA amplification
• In situ genotyping with padlock probes
Recombination based-cloning
• Cell-free cloning of lethal DNA
• RNA-primed DNA amplification


Addition of Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis.

Use of this enzyme in certain applications may be covered by patents and may require a license.
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: phi29 DNA Polymerase
Product Size: 250 units


Manuals & protocols