Terminal Deoxynucleotidyl Transferase (20 U/μL)
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Terminal Deoxynucleotidyl Transferase (20 U/μL)
Thermo Scientific™

Terminal Deoxynucleotidyl Transferase (20 U/μL)

Thermo Scientific Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes the repetitive addition of deoxyribonucleotides to theRead more
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Catalog NumberQuantity
EP0161500 U
EP01622500 U
2 Options
Catalog number EP0161
Price (USD)/ 500 units
82.65
Online exclusive
98.25 
Save 15.60 (16%)
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Add to cart
Quantity:
500 U
Request bulk or custom format
Price (USD)/ 500 units
82.65
Online exclusive
98.25 
Save 15.60 (16%)
Add to cart
Terminal Deoxynucleotidyl Transferase (20 U/μL)
Catalog numberEP0161
Price (USD)/ 500 units
82.65
Online exclusive
98.25 
Save 15.60 (16%)
-
Add to cart
Thermo Scientific Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA. TdT requires an oligonucleotide of at least three nucleotides to serve as a primer. With RNA as template TdT shows variable performance which strongly depends upon the tertiary structure of acceptor RNA 3'-end and the nature of nucleotide. Generally, it is lower than using DNA as a template.

Highlights

Incorporates modified nucleotides (e.g., fluorescein-, biotin-, aminoallyl-labeled nucleotides)

Applications

• Production of synthetic homo- and heteropolymers
• Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus
• Oligodeoxyribonucleotide and DNA labeling
• 5'-RACE (Rapid Amplification of cDNA Ends)
In situ localization of apoptosis

Note

Due to the presence of CoCl2, the TdT Reaction Buffer is incompatible with downstream applications. It is necessary to remove CoCl2 from the reaction mixture by spin column or phenol/chloroform extraction and subsequent ethanol precipitation.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration20 U/μL
EnzymeTerminal Deoxynucleotidyl Transferase
Compatible BufferStorage Buffer, 5X Reaction Buffer
Quantity500 U
Product TypeTdT
Unit Size500 units