Maxima H Minus Reverse Transcriptase (200 U/μL)
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Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific™

Maxima H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possessesRead more
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Catalog NumberQuantity
EP075210,000 Units
EP07512,000 Units
EP07534 x 10,000 Units
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Catalog number EP0752
Price (USD)/ 10,000 units
216.00
Special Offer
Online exclusive
Ends: 16-Dec-2024
270.00 
Save 54.00 (20%)
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Quantity:
10,000 Units
Request bulk or custom format
Price (USD)/ 10,000 units
216.00
Special Offer
Online exclusive
Ends: 16-Dec-2024
270.00 
Save 54.00 (20%)
Add to cart
Maxima H Minus Reverse Transcriptase (200 U/μL)
Catalog numberEP0752
Price (USD)/ 10,000 units
216.00
Special Offer
Online exclusive
Ends: 16-Dec-2024
270.00 
Save 54.00 (20%)
-
Add to cart

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT. The engineered enzyme features dramatically improved thermostability, 50X higher processivity, robustness, and increased synthesis rate compared to wild type M-MuLV RT.

The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors, such as guanidine, formamide, and ethanol. 

Features of Maxima H Minus Reverse Transcriptase include:
• Thermostable—90% active after incubation at 50°C for 60 minutes in a reaction mixture
• Active up to 65°C
• High yields of full-length cDNA up to 20 kb
• High sensitivity—reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 μg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Increased resistance to common reaction inhibitors
• Incorporates modified nucleotides

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• Analysis of RNA by primer extension

Related products
Maxima H Minus Reverse Transcriptase
Maxima H Minus First Strand cDNA Synthesis Kit
Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase
Maxima H Minus cDNA Synthesis Master Mix
Maxima H Minus cDNA Synthesis Master Mix, with dsDNase

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone Enzyme
GC-Rich PCR PerformanceHigh
Reaction Speed15 to 30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C to 55°C
Quantity10,000 Units
Reverse TranscriptaseMaxima H Minus
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Concentration200 U/μL
Final Product TypeFirst-Strand cDNA
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
Unit Size10,000 units
Contents & Storage

• Maxima H Minus Reverse Transcriptase, 1 x 10,000 units (200 U/μL)
• 5X RT Buffer

Store at –20°C.