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Thermo Scientific™

Esp3I (BsmBI) (10 U/μL)

Catalog number: ER0451
Thermo Scientific™

Esp3I (BsmBI) (10 U/μL)

Catalog number: ER0451
Catalog Number
ER0451
Unit Size
200 units
Price (USD)
Price: 101.00
Online price: 85.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
1,000 units
Price (USD)
Price: 398.00
Online price: 361.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
ER0451200 units
Price: 101.00
Online price: 85.65
Your price:
-
ER04521,000 units
Price: 398.00
Online price: 361.65
Your price:
-
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5'  C  G  T  C  T  C  N1 3'
3'  G  C  A  G  A  G  N5 5'

Thermo Scientific Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: BsmBI.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that sent in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: The enzyme requires DTT. Freshly made DTT should be added to the reaction buffer. Esp3I cleaves downstream of its recognition site and can generate any desired 4 base 5'-overhangs. This feature is useful for PCR product cloning. For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
10 U/μL
Methylation Sensitivity
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme
Esp3I (BsmBI)
Compatible Buffer
10X Buffer Tango
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
Yes
Quantity
200 U
Product Type
Restriction Enzyme
Research Category
Scarless Cloning

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