Thermo Scientific™

SalI (10 U/µL)

Catalog number: ER0641
Thermo Scientific™

SalI (10 U/µL)

Catalog number: ER0641
Catalog Number
ER0641
Unit Size
1,500 units
Price (USD)
57.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 x 1,500 units
Price (USD)
216.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
2,000 units
Price (USD)
76.50
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
ER06411,500 units
57.00
Your Price:
***
ER06425 x 1,500 units
216.00
Your Price:
***
ER06452,000 units
76.50
Your Price:
***
Product Overview
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5'  G ↓T  C  G  A  C   3' 
3'  C  A  G  C  T ↑G   5' 

Thermo Scientific SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: Incubation at 25°C results in 50-75% activity. Low salt, high glycerol (> 5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity. Supercoiled forms of pBR322 and pUC require 10-fold overdigestion with SalI to achieve complete digestion. For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Methylation Sensitivity
Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive
Enzyme
Sal I
Compatible Buffer
10X Buffer O
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
37°C
Type IIS RE
No

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Frequently asked questions (FAQs)

Citations & References