SchI (MlyI) (10 U/μL)
SchI (MlyI) (10 U/μL)
SchI (MlyI) (10 U/μL)
SchI (MlyI) (10 U/μL)
Thermo Scientific™

SchI (MlyI) (10 U/μL)

5' G A G T C N5↓ 3'3' C T C A G N5↑ 5'Thermo Scientific SchI (MlyI) restriction enzymeRead more
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Catalog NumberQuantity
ER13711,000 units
Catalog number ER1371
Price (USD)/ Each
117.00
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Quantity:
1,000 units
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Price (USD)/ Each
117.00
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5'  G  A  G  T  C  N5 3'
3'  C  T  C  A  G  N5 5'

Thermo Scientific SchI (MlyI) restriction enzyme recognizes GAGTC(5/5)^ sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: MlyI, PleI, PpsI, SchI.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: Greater than 10-fold overdigestion with SchI may result in star activity. SchI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis. For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible Buffer10X Buffer Tango
Product TypeRestriction Enzyme
Quantity1,000 units
Concentration10 U/μL
EnzymeSchI (MlyI)
Methylation SensitivityNot Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Optimal Reaction Temperature37°C
Research CategoryTraditional Cloning
Sensitive to Heat InactivationYes
Type IIS REYes
Unit SizeEach