Thermo Scientific™

SmoI (SmlI) (10 U/µL)

Catalog number: ER1981
Thermo Scientific™

SmoI (SmlI) (10 U/µL)

Catalog number: ER1981
Catalog Number
ER1981
Unit Size
200 units
Price (USD)
74.25
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
ER1981200 units
74.25
Your Price:
***
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5'  C ↓T  Y  R  A  G   3' 
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Thermo Scientific SmoI (SmlI) restriction enzyme recognizes C^TYRAG sites and cuts best at 55°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Isoschizomers: SmlI.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: Incubation at 37°C results in 10% activity. SmoI cleavage is impaired by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain such as GM2163.Low salt, high glycerol (>5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity. For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Methylation Sensitivity
Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive
Enzyme
SmoI (SmlI)
Compatible Buffer
10X Buffer Tango
Sensitive to Heat Inactivation
Yes
Optimal Reaction Temperature
55°C
Type IIS RE
No

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