SmoI (SmlI) (10 U/µL)

Catalog number: ER1981

Thermo Scientific™  Related applications: Restriction Enzyme Cloning

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5'  C ↓T  Y  R  A  G   3' 
3'  G  A  R  Y  T ↑C   5' 

Thermo Scientific SmoI (SmlI) restriction enzyme recognizes C^TYRAG sites and cuts best at 55°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Isoschizomers: SmlI.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.


• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities


• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)

Note: Incubation at 37°C results in 10% activity. SmoI cleavage is impaired by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain such as GM2163.Low salt, high glycerol (>5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity. For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.


Enzyme: SmoI (SmlI)
Product Size: 200 units
Compatible Buffer: 10x Buffer Tango
Methylation Sensitivity: Not CpG methylation-sensitive, Not dcm methylation-sensitive, dam methylation-sensitive
Optimal Reaction Temperature: 55° C
Sensitive to Heat Inactivation: Yes


Manuals & protocols

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