IFN gamma ELISA Reagent Kit, Bovine

Catalog number: ESS0026B

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Assay IFN-γ (bovine interferon gamma) levels in cow cell culture supernatant samples with our Thermo Scientific Bovine IFN gamma ELISA Reagent Kit.

Features of the Bovine IFN gamma ELISA Reagent Kit:

Target: natural and recombinant bovine interferon gamma (IFNγ)
Format: colorimetric ELISA (TMB substrate) for 96-well microplates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 31.2 to 2000 pg/mL
Sensitivity: undetermined
Sample Types: cell culture supernatant
Total Assay Time: 3 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with recombinant bovine IL-1β, IL-2, IL-4, IL-6, IL-8, or TNFα, or with recombinant human, mouse, or rat IFNγ.

This bovine IFN-γ ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:

The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Cattle⁄Bovine
Product Size: 480 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols