IL-6 Bovine Uncoated ELISA Kit

Catalog number:  ESS0029

 Related applications: Protein Assays and Analysis

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Assay IL-6 (bovine interleukin-6) levels in bovine cell culture supernatant samples with our Thermo Scientific Bovine IL-6 ELISA Reagent Kit.

Features of the Bovine IL-6 ELISA Reagent Kit:

Target: natural and recombinant bovine interleukin-6 (IL-6)
Format: colorimetric ELISA (TMB substrate) for 96-well microplates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 78 to 5000 pg/mL
Sensitivity: undetermined
Sample Types: cell culture supernatant
Total Assay Time: 3.5 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with recombinant bovine IL-1β, IL-2, IL-4, IL-8, IFNγ, or TNFα.

This bovine IL-6 ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.


• IL-6 ELISA Kit literature references
For Research Use Only. Not for use in diagnostic procedures.


Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene ID: 280826
Gene Name: interleukin 6
Gene Symbol: IL6
Immunoassay Kit Format: Uncoated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 5 x 96 tests
Protein Aliases: IL-6
Protein Name: Interleukin-6
Target (Kit Named): IL-6
Target Species (Named): Bovine
Target Species (Validated): Bovine
UniProt ID: P26892

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols