IL-4 Bovine Uncoated ELISA Kit

Catalog number: ESS0031

Invitrogen™  Related applications: Protein Assays and Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Assay IL-4 (bovine interleukin-4) levels in bovine cell culture supernatant samples with our Thermo Scientific Bovine IL-4 ELISA Reagent Kit.

Features of the Bovine IL-4 ELISA Reagent Kit:

Target: natural and recombinant bovine interleukin-4 (IL-4)
Format: colorimetric ELISA (TMB substrate) for 96-well microplates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 16 to 1000 pg/mL
Sensitivity: undetermined
Sample Types: cell culture supernatant
Total Assay Time: 3.5 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with recombinant bovine IL-1β, IL-2, IL-6, IL-8, IFNγ or TNFα no significant cross-reaction with recombinant human, equine, mouse, porcine, rat, or rhesus macaque IL-4.

This bovine IL-4 ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-4 (IL-4) in samples at 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Cytokines & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: BSF-1, IL-4
Gene ID: 280824
Gene Name: interleukin 4
Gene Symbol: IL4
Immunoassay Kit Format: Uncoated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 5 x 96 tests
Protein Aliases: IL-4, B-cell stimulatory factor 1, BSF-1, Lymphocyte stimulatory factor 1
Protein Name: Interleukin-4
Target (Kit Named): IL-4
Target Species (Named): Bovine
Target Species (Validated): Bovine
UniProt ID: P30367

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols