IL-4 ELISA Reagent Kit, Bovine

Catalog number: ESS0031

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Assay IL-4 (bovine interleukin-4) levels in bovine cell culture supernatant samples with our Thermo Scientific Bovine IL-4 ELISA Reagent Kit.

Features of the Bovine IL-4 ELISA Reagent Kit:

Target: natural and recombinant bovine interleukin-4 (IL-4)
Format: colorimetric ELISA (TMB substrate) for 96-well microplates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 16 to 1000 pg/mL
Sensitivity: undetermined
Sample Types: cell culture supernatant
Total Assay Time: 3.5 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with recombinant bovine IL-1β, IL-2, IL-6, IL-8, IFNγ or TNFα no significant cross-reaction with recombinant human, equine, mouse, porcine, rat, or rhesus macaque IL-4.

This bovine IL-4 ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-4 (IL-4) in samples at 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.
For Research Use Only. Not for use in diagnostic procedures.


Species: Cattle⁄Bovine
Product Size: 480 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols