Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Thermo Scientific™

Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
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Catalog NumberColorNo. of Reactions
F-530XLColorless2000 Reactions
F530SColorless100 Reactions
F530LColorless500 Reactions
F534SGreen100 Reactions
F534LGreen500 Reactions
Catalog number F-530XL
Price (EUR)
1 560,65
Online Exclusive
1 924,00
Save 363,35 (19%)
Each
Add to cart
Color:
Colorless
No. of Reactions:
2000 Reactions
Request bulk or custom format
Price (EUR)
1 560,65
Online Exclusive
1 924,00
Save 363,35 (19%)
Each
Add to cart
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorColorless
Fidelity (vs. Taq)52X
Hot StartNo
No. of Reactions2000 Reactions
OverhangBlunt
PolymerasePhusion High Fidelity DNA Polymerase
Product TypeHigh-Fidelity DNA Polymerase
Quantity2000 units
Reaction FormatStandalone
Shipping ConditionDry Ice
Size (Final Product)20 kb or less
Concentration2 U/μL
For Use With (Application)Standard PCR, High-fidelity PCR
GC-Rich PCR PerformanceHigh
Reaction SpeedFast
Unit SizeEach
Contents & Storage
• Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution

Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.