Tinción de mielina fluorescente FluoroMyelin™ roja: solución en agua

Citas y referencias (14)

Invitrogen™

Tinción de mielina fluorescente FluoroMyelin™ roja: solución en agua

La tinción fluorescente de mielina FluoroMyelin™ Red permite un etiquetado rápido y selectivo de la mielina en cortes criogénicos cerebralesMás información

Número de catálogo	Cantidad
F34652	1 mL

Número de catálogo F34652

Precio (MXN)

Cantidad:

La tinción fluorescente de mielina FluoroMyelin™ Red permite un etiquetado rápido y selectivo de la mielina en cortes criogénicos cerebrales en un solo paso de etiquetado de 20 minutos más lavados. Esta tinción se puede utilizar junto con anticuerpos y otros colorantes, y con métodos histoquímicos estándar para el material de cortes criogénicos.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorRojo

Método de detecciónFluorescencia

Para utilizar con (equipo)Microscopio de fluorescencia

Etiqueta o tinteFluoroMyelin™ Red

Tipo de productoTinción de mielina roja fluorescente

Cantidad1 mL

Condiciones de envíoTemperatura ambiente

Localización subcelularMielina

Excitation/Emission558/654 nm

Línea de productosFluoroMyelin

Unit SizeEach

Contenido y almacenamiento

• Se suministra como una concentración de 300X en agua.
• Cuando se almacena a 4 °C, se mantiene estable durante al menos 6 meses después de su recepción.
• Proteger de la luz.

Preguntas frecuentes

I used one of your FluoroMyelin stains and noticed it stains cells other than glial cells. Is there something wrong with the product?

FluoroMyelin is a lipid stain, any lipid can be stained by it but there is a higher lipid content in myelin that it will stain much more intensely than other membranes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the FluoroMyelin stains be used on neurons in culture?

We have only tested them on mouse brain cryosections. They might work on neurons in culture, but would likely stain the membrane and there would be some endocytosis of the stains. Myelin sheaths may show enhanced intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the FluoroMyelin myelin stains be used on paraffin sections?

We have only tested them on mouse brain cryosections.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the FluoroMyelin myelin stains?

They are proprietary lipid stains that stain myelin, but are not neuron specific.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (14)

Citations & References

Abstract

Prion protein (PrPc) positively regulates neural precursor proliferation during developmental and adult mammalian neurogenesis.

Authors:Steele AD, Emsley JG, Ozdinler PH, Lindquist S, Macklis JD

Journal:Proc Natl Acad Sci U S A

PubMed ID:16492732

'The misfolding of the prion protein (PrP(c)) is a central event in prion diseases, yet the normal function of PrP(c) remains unknown. PrP(c) has putative roles in many cellular processes including signaling, survival, adhesion, and differentiation. Given the abundance of PrP(c) in the developing and mature mammalian CNS, we investigated ... More

Rescuing qkV dysmyelination by a single isoform of the selective RNA-binding protein QKI.

Authors:Zhao L, Tian D, Xia M, Macklin WB, Feng Y,

Journal:J Neurosci

PubMed ID:17079655

Alternative splicing of the qkI transcript generates multiple isoforms of the selective RNA-binding protein QKI, which play key roles in controlling the homeostasis of their mRNA targets. QKI deficiency in oligodendrocytes of homozygous quakingviable (qkV/qkV) mutant mice results in severe hypomyelination, indicating the essential function of QKI in myelinogenesis. However, ... More

Neuronal glutathione deficiency and age-dependent neurodegeneration in the EAAC1 deficient mouse.

Authors:Aoyama K, Suh SW, Hamby AM, Liu J, Chan WY, Chen Y, Swanson RA

Journal:Nat Neurosci

PubMed ID:16311588

Uptake of the neurotransmitter glutamate is effected primarily by transporters expressed on astrocytes, and downregulation of these transporters leads to seizures and neuronal death. Neurons also express a glutamate transporter, termed excitatory amino acid carrier-1 (EAAC1), but the physiological function of this transporter remains uncertain. Here we report that genetically ... More

Collagen XXVIII, a novel von Willebrand factor A domain-containing protein with many imperfections in the collagenous domain.

Authors:Veit G, Kobbe B, Keene DR, Paulsson M, Koch M, Wagener R

Journal:J Biol Chem

PubMed ID:16330543

Here we describe a novel collagen belonging to the class of von Willebrand factor A (VWA) domain-containing proteins. This novel protein was identified by screening the EST data base and was subsequently recombinantly expressed and characterized as an authentic tissue component. The COL28A1 gene on human chromosome 7p21.3 and on ... More

Skin and gut imprinted helper T cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity.

Authors:

Journal:Nat Immunol

PubMed ID:34099917

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