Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:
• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7
) is lower than that in GC Buffer (9.5 × 10-7
). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.
5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.Related ProductsPhusion GC Buffer Pack, detergent-freePhusion HF Buffer Pack, detergent-freePhusion HF Buffer PackPhusion Green HF Buffer PackPhusion Green GC Buffer Pack
For Research Use Only. Not for use in diagnostic procedures.