Thermo Scientific™

Phire Reaction Buffer, detergent-free

Catalog number:  F525L

Related applications:


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The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents (F-524L and F-525L, respectively) or Green version (F-527L) containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The concentration of Mg2+ in the reaction buffers is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.

Related Products
Phire Green Reaction Buffer
Phire Reaction Buffer
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Phire Hot Start II DNA polymerase
Product Line: Phire
Product Size: 4 x 1.5 mL

Contents & storage

• 4 x 1.5 mL 5X Phire Reaction Buffer, detergent-free (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
Store at -20°C.