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Catalog Number
F527L
also known as F-527L
Unit Size
4 x 1.5 mL
Price (USD)
Price: 36.64
Your price:
Availability
-
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| F527L also known as F-527L | 4 x 1.5 mL | Price: 36.64 Your price: | - |
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The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents (F-524L and F-525L, respectively) or Green version (F-527L) containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
The concentration of Mg2+ in the reaction buffers is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.
Related Products
Phire Reaction Buffer
Phire Reaction Buffer, detergent-free
The concentration of Mg2+ in the reaction buffers is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.
Related Products
Phire Reaction Buffer
Phire Reaction Buffer, detergent-free
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Polymerase
Phire Hot Start II DNA polymerase
Product Line
Phire
Product Type
PCR Reaction Buffer
Concentration
5X
Volume
4 x 1.5 mL
Contents & Storage
Contains:
• 4 x 1.5 mL 5X Phire Green Reaction Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO
Store at -20°C.
• 4 x 1.5 mL 5X Phire Green Reaction Buffer (1X buffer provides 1.5 mM MgCl2)
• 50 mM MgCl2
• DMSO
Store at -20°C.
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