Phusion High-Fidelity DNA Polymerase (2 U/µL)

Catalog number: F530L

Thermo Scientific™  Related applications: PCR

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Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.


• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)


• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Phusion High-Fidelity DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 52 X
GC-Rich PCR Performance: High
Reaction Format: Standalone
Product Overhang: Blunt
Reaction Speed: Fast
Product Size: 500 units

Contents & storage

• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• 50 mM MgCl2 solution

Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

Store at -20°C.


Manuals & protocols

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