Informational:Larger pack sizes of Phusion High-Fidelity DNA Polymerase with dNTPs now available.
Thermo Scientific™

Phusion™ High-Fidelity DNA Polymerase (2 U/µL)

Catalog number: F530L
Thermo Scientific™

Phusion™ High-Fidelity DNA Polymerase (2 U/µL)

Catalog number: F530L
Catalog Number
F530L
also known as F-530L
Unit Size
500 units
Price (USD)
Price: 489.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
2000 units
Price (USD)
Price: 1,758.00
Your Price:
Availability
***
Quantity
Catalog Number
F530S
also known as F-530S
Unit Size
100 units
Price (USD)
Price: 121.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F530L
also known as F-530L
500 units
Price: 489.00
Your Price:
***
F-530XL2000 units
Price: 1,758.00
Your Price:
***
F530S
also known as F-530S
100 units
Price: 121.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Highlights

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
2 U/µL
Fidelity (vs. Taq)
52 X
Format
Stand-alone enzyme
GC-Rich PCR Performance
High
Hot Start
No
No. of Reactions
500
Overhang
Blunt
Polymerase
Phusion High-Fidelity DNA Polymerase
Reaction Format
Standalone
Reaction Speed
Fast
Size (Final Product)
20 kb or Less
Starting Material
DNA
Includes
Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration.
Product Line
Phusion
Product Type
High-Fidelity DNA Polymerase
Quantity
500 U (2 U/μL)
For Use With (Application)
PCR Amplification

Contents & Storage

Includes
• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution

Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

Store at -20°C.

Figures