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Informational:Larger pack sizes of Phusion High-Fidelity DNA Polymerase with dNTPs now available.
Thermo Scientific™

Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Catalog number: F530S
Thermo Scientific™

Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Catalog number: F530S
Catalog Number
F530S
Unit Size
100 units
Price (USD)
Price: 132.00
Online price: 104.65
Your price:
Availability
-
Quantity
Catalog Number
F530L
also known as F-530L
Unit Size
500 units
Price (USD)
Price: 532.00
Online price: 435.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
2000 units
Price (USD)
Price: 1,910.00
Online price: 1,508.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F530S100 units
Price: 132.00
Online price: 104.65
Your price:
-
F530L
also known as F-530L
500 units
Price: 532.00
Online price: 435.65
Your price:
-
F-530XL2000 units
Price: 1,910.00
Online price: 1,508.65
Your price:
-
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Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

Features
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15–30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

GC-Rich PCR Performance
High
Polymerase
Phusion High-Fidelity DNA Polymerase
Reaction Speed
Fast
Product Type
High-Fidelity DNA Polymerase
Quantity
100 U
Shipping Condition
Dry Ice
For Use With (Application)
Standard PCR, High-fidelity PCR
Concentration
2 U/μL
Fidelity (vs. Taq)
52X
Hot Start
No
No. of Reactions
100 Reactions
Overhang
Blunt
Reaction Format
Standalone
Size (Final Product)
20 kb or less

Contents & Storage

• Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution

Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.

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