Image-iT™ 4% Formaldehyde Fixative Solution in PBS (methanol-free), 20 mL - FAQs

View additional product information for Image-iT™ Fixative Solutions - FAQs (R37814, I28700, I28800, I28900, FB002)

4 product FAQs found

The Image-iT Fixation/Permeabilization Kit includes Triton X-100 as the permeabilizing reagent. May I substitute other reagents for permeabilizing?

Yes. Aldehyde-based fixation is compatible with other permeabilizing reagents, such as saponin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the Image-iT Fixative Solution alcohol-free?

Yes, it is alcohol-free.

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Can I add the Image-iT Fixative Solution in with the media?

We do not recommend doing this. The Image-iT Fixative Solution is 4% formaldehdye in PBS, so mixing it in directly with media would dilute the final concentration of fixative and this may result in less than optimal fixation. If you need to add fixative directly to the medium, consider using a fixative at a higher concentration such as formaldehyde at 16% (Cat. No. 28906) or at 37% (Cat. No. 9311).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What fixative should I use to fix my cells or tissues for antibody labeling?

Aldehyde-based fixatives (e.g., formaldehyde, glutaraldehyde) crosslink various cellular components, which helps retain proteins and cell morphology, but some antigens can be masked by the crosslinking, requiring antigen retrieval methods to unmask the antigen binding sites. Also, aldehyde-based fixation requires permeabilization to allow entry of antibodies. Organic solvents such as methanol and acetone condense proteins and permeabilize the cells, but cell morphology can be compromised. Surface antigens can be labeled without fixation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.