Quantitative assessment of in planta distribution of metabolic activity and gene expression of an endophytic fungus.
AuthorsHerd S, Christensen MJ, Saunders K, Scott DB, Schmid J
JournalMicrobiology
PubMed ID10627142
Using perennial ryegrass infected with an Acremonium transformant carrying the Escherichia coli beta-D-glucuronidase gene (gusA) (GUS system) under control of a constitutive promoter, we have developed methods for the quantitative extraction of endophyte-associated GUS activity from plant material. Fluorometric assays of these extracts allow quantitative assessment of the distribution of ... More
Nonablative neonatal marrow transplantation attenuates functional and physical defects of beta-glucuronidase deficiency.
AuthorsSoper BW, Lessard MD, Vogler CA, Levy B, Beamer WG, Sly WS, Barker JE
JournalBlood
PubMed ID11222399
The toxicity of preparative regimens render neonatal bone marrow transplantation (BMT) for progressive childhood diseases a controversial treatment. Ablative BMT in neonatal mice with or without the lysosomal storage disease mucopolysaccharidosis type VII (MPS VII) show high morbidity and developmental disruption of both brain and bone structure. In this investigation, ... More
Fluorescent imaging of GUS activity and RT-PCR analysis of gene expression in the shoot apical meristem.
AuthorsFleming AJ, Manzara T, Gruissem W, Kuhlemeier C
JournalPlant J
PubMed ID8893550
The use of promoter-reporter gene constructs in transgenic plants is a powerful tool in the analysis of gene expression which can, however, be limited in the resolution of small structures, such as the apical meristem. This paper reports on a fluorescent imaging technique for the analysis of GUS reporter gene ... More