Cell fusion-independent differentiation of neural stem cells to the endothelial lineage.
AuthorsWurmser AE, Nakashima K, Summers RG, Toni N, D'Amour KA, Lie DC, Gage FH
JournalNature
PubMed ID15254537
'Somatic stem cells have been claimed to possess an unexpectedly broad differentiation potential (referred to here as plasticity) that could be induced by exposing stem cells to the extracellular developmental signals of other lineages in mixed-cell cultures. Recently, this and other experimental evidence supporting the existence of stem-cell plasticity have ... More
Early activation, motility, and homing of neonatal microglia to injured neurons does not require protein synthesis.
AuthorsKurpius D, Wilson N, Fuller L, Hoffman A, Dailey ME
JournalGlia
PubMed ID16715500
'Neuronal injury in CNS tissues induces a rapid activation and mobilization of resident microglia (MG). It is widely assumed that changes in gene expression drive the morphological transformation of MG and regulate their mobilization during activation. Here, we used acutely excised neonatal rat brain slices to test whether the morphological ... More
Phenotypic characterization of gastric sensory neurons in mice.
AuthorsBielefeldt K, Zhong F, Koerber HR, Davis BM,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID16728726
Recent studies suggest that the capsaicin receptor [transient receptor potential vanilloid (TRPV)1] may play a role in visceral mechanosensation. To address the potential role of TRPV1 in vagal sensory neurons, we developed a new in vitro technique allowing us to determine TRPV1 expression directly in physiologically characterized gastric sensory neurons. ... More
Sequences in intron 51 of the von Willebrand factor gene target promoter activation to a subset of lung endothelial cells in transgenic mice.
AuthorsKleinschmidt AM, Nassiri M, Stitt MS, Wasserloos K, Watkins SC, Pitt BR, Jahroudi N,
JournalJ Biol Chem
PubMed ID18048367
In vivo analyses of the VWF promoter previously demonstrated that a fragment spanning sequences -487 to +247 targets promoter activation to brain vascular endothelial cells, whereas a longer fragment including 2182 bp of the 5'-flanking sequences, the first exon, and the first intron activated expression in endothelial cells of the ... More
A new method to isolate microglia from adult mice and culture them for an extended period of time.
AuthorsMoussaud S, Draheim HJ,
JournalJ Neurosci Methods
PubMed ID20097228
As the major immuno-competent cells of the brain, microglia are highly implicated in neuro-protection as well as in neurodegeneration. Therefore, they are of key interest for research on numerous CNS diseases. Currently, to model inflammation in the brain, microglial cell lines or primary microglia prepared from embryonic or neo-natal rodents ... More
Microglia promote colonization of brain tissue by breast cancer cells in a Wnt-dependent way.
AuthorsPukrop T, Dehghani F, Chuang HN, Lohaus R, Bayanga K, Heermann S, Regen T, Van Rossum D, Klemm F, Schulz M, Siam L, Hoffmann A, Trümper L, Stadelmann C, Bechmann I, Hanisch UK, Binder C,
JournalGlia
PubMed ID20549749
Although there is increasing evidence that blood-derived macrophages support tumor progression, it is still unclear whether specialized resident macrophages, such as brain microglia, also play a prominent role in metastasis formation. Here, we show that microglia enhance invasion and colonization of brain tissue by breast cancer cells, serving both as ... More
Microglial cells in astroglial cultures: a cautionary note.
AuthorsSaura J,
JournalJ Neuroinflammation
PubMed ID17937799
Primary rodent astroglial-enriched cultures are the most popular model to study astroglial biology in vitro. From the original methods described in the 1970's a great number of minor modifications have been incorporated into these protocols by different laboratories. These protocols result in cultures in which the astrocyte is the predominant ... More
Variational autoencoding of gene landscapes during mouse CNS development uncovers layered roles of Polycomb Repressor Complex 2.
Authors
JournalNucleic Acids Res
PubMed ID35048973
The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn.
Authors
JournalCell
PubMed ID28041852
PIEZO1 transduces mechanical itch in mice.
Authors
JournalNature
PubMed ID35732741
Parallel ascending spinal pathways for affective touch and pain.
Authors
JournalNature
PubMed ID33116307
Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors.
Authors
JournalNat Commun
PubMed ID32170060
Blood flow drives lumen formation by inverse membrane blebbing during angiogenesis in vivo.
Authors
JournalNat Cell Biol
PubMed ID26928868
Epicardium-derived cells organize through tight junctions to replenish cardiac muscle in salamanders.
Authors
JournalNat Cell Biol
PubMed ID35550612
A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis.
Authors
JournalNat Commun
PubMed ID33972531
Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity.
Authors
JournalNat Cell Biol
PubMed ID25730471
Exercise-induced angiogenesis is dependent on metabolically primed ATF3/4+ endothelial cells.