Luminaris Color HiGreen qPCR Master Mix

Catalog number: K0392

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).


• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection


Luminaris Color HiGreen qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye; supplied with 40X Yellow Sample Buffer and nuclease-free water.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: SYBR
For Use With (Equipment): Cepheid SmartCycler, MJ Opticon, BioRad CFX96™, Roche LightCycler 480, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Corbett RotorGene, MJ Chromo4
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): dsDNA, DNA
Polymerase: Taq DNA Polymerase
Product Size: 500 reactions
Number of Reactions: 500 reactions

Contents & storage

• 4 x 1.25 mL Luminaris Color HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye; sufficient for 500 x 20 µL reactions
• 1 x 1.25 mL 40X Yellow Sample Buffer
• 4 x 1.25 mL Nuclease-Free Water

Store at -20°C.


Manuals & protocols

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