Thermo Scientific™

TranscriptAid T7 High Yield Transcription Kit

Catalog number:  K0441

 Related applications:

In Vitro Transcription

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Thermo Scientific TranscriptAid T7 High Yield Transcription Kit contains reagents for 50 reactions of 20 µL. Each reaction yields up to 200 µg RNA from 1 µg of template in 2 hours. The reaction can be scaled up to produce milligram amounts of full-length RNA. The kit can be used to produce both long and short transcripts for applications that require large yields of RNA. All necessary reagents for transcription are included, as well as the RiboRuler High Range RNA Ladder for sizing and quantification.


Exceptionally high yields—up to 200 µg in 2 hours
Versatile—suitable for both short and long RNA transcripts
Milligram amounts of RNA in a single, scaled-up reaction
Flexible—generates unlabeled, labeled or capped RNA
RiboRuler RNA Ladder supplied with kit for sizing and quantification


In vitro transcription
In vitro translation
• Generation of hybridization probes for:
• microarrays
in situ hybridization
• blotting

• RNase protection assays
• RNA binding protein assays
• Antisense RNA and RNAi
• RNA amplification
• Microinjection studies


• TranscriptAid Enzyme Mix
• 5X TranscriptAid Reaction Buffer
• DNase I, RNase-free
ATP Solution
CTP Solution
GTP Solution
UTP Solution
• Control template
• 3 M Sodium Acetate Solution, pH 5.2
• DEPC-treated Water
• 2X RNA Loading Dye
RiboRuler High Range RNA Ladder, ready-to-use
• 0.5 M EDTA, pH 8.0
• Detailed Protocol

* The improved version of TranscriptAid T7 High Yield Transcription Kit contains Tris-buffered NTPs instead of previously used NTPs titrated with KOH. Usage of Tris-buffered NTPs leads to higher yields of RNA transcripts.
For Research Use Only. Not for use in diagnostic procedures.


Starting Material: DNA
Promoter: T7
RNA Yield: Up to 200 µg
Final Product: In Vitro Transcribed RNA
Product Line: TranscriptAid
Product Size: 50 reactions


Manuals & protocols