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Maxima First Strand cDNA Synthesis Kit for RT-qPCR

Catalog number: K1641

Thermo Scientific™  Related applications: cDNA Libraries & Library Construction

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Description

Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR is a convenient system optimized for cDNA synthesis in 2-step quantitative RT-PCR (RT-qPCR) applications. The kit uses Maxima Reverse Transcriptase (RT), an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT.

The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 µg) at elevated temperatures (42 to 65°C).The synthesis reaction can be completed in 15 to 30 minutes. Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors.

Highlights

• High yields of full length cDNA up to 20 kb
• Efficient cDNA synthesis at wide temperature range from 42°C to 65°C
• Increased synthesis rate—complete cDNA synthesis in 15-30 minutes
• High sensitivity and specificity

Applications

• 2-step RT-PCR
• 2-step RT-qPCR

Maxima First Strand cDNA Synthesis Kit for RT-qPCR contains
• Maxima Enzyme Mix
• 5X Reaction Mix
• nuclease-free water

Additional Information about Reaction Components
The Maxima Enzyme Mix contains Maxima Reverse Transcriptase and Thermo Scientific RiboLock RNase Inhibitor,. The recombinant RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B and C at temperatures up to 55°C.

5X Reaction Mix contains the remaining reaction components: reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers.

Water, nuclease-free is provided for reaction set-up and dilution of sample RNA. The absence of endo-, exodeoxyribonucleases, ribonucleases and phosphatases has been confirmed by appropriate quality tests.

Related Products
Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase
Maxima First Strand cDNA Synthesis Kit for RT-qPCR
Maxima First Strand cDNA Synthesis Kit Reaction Mix (5X)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reverse Transcriptase: Maxima Reverse Transcriptase
Downstream Application: Real-Time Quantitative PCR (qPCR)
Reaction Format: SuperMix or Master Mix
Sample Type (General): RNA
Optimal Reaction Temperature: 50-55° C
Product Line: Maxima
Product Size: 50 reactions

Contents & storage

Maxima First Strand cDNA Synthesis Kit for RT-qPCR contains:
• Maxima Enzyme Mix (Maxima Reverse Transcriptase and RiboLock RNase Inhibitor)
• 5X Reaction Mix (reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers)
• nuclease-free water

Store at -20°C.

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Manuals & protocols

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