Semaphorin III can function as a selective chemorepellent to pattern sensory projections in the spinal cord.
AuthorsMessersmith EK, Leonardo ED, Shatz CJ, Tessier-Lavigne M, Goodman CS, Kolodkin AL
JournalNeuron
PubMed ID7748562
'Distinct classes of primary sensory neurons in dorsal root ganglia subserve different sensory modalities, terminate in different dorsoventral locations in the spinal cord, and display different neurotrophin response profiles. Large diameter muscle afferents that terminate in the ventral spinal cord are NT-3 responsive, whereas small diameter afferents subserving pain and ... More
Kainate receptor subunits expressed in single cultured hippocampal neurons: molecular and functional variants by RNA editing.
To determine the kainate receptor subunits that are found in native kainate receptors, we have applied a multiplex PCR of cDNAs reverse transcribed from mRNA harvested from single cultured hippocampal neurons after electrophysiological recording. We found that all the cells showing rapidly desensitizing currents in response to kainate express the ... More
Map-based cloning of chloronerva, a gene involved in iron uptake of higher plants encoding nicotianamine synthase.
AuthorsLing HQ, Koch G, Baumlein H, Ganal MW
JournalProc Natl Acad Sci U S A
PubMed ID10359845
The uptake of iron in plants is a highly regulated process that is induced on iron starvation. In tomato, the mutant chloronerva exhibits constitutive expression of iron uptake responses and intercostal chlorosis. Biochemically, chloronerva is an auxotroph for nicotianamine, a key polyamine in plant iron uptake metabolism. The chloronerva gene ... More
ARIA, a protein that stimulates acetylcholine receptor synthesis, is a member of the neu ligand family.
AuthorsFalls DL, Rosen KM, Corfas G, Lane WS, Fischbach GD
JournalCell
PubMed ID8453670
Motor neurons stimulate their postsynaptic muscle targets to synthesize neurotransmitter receptors. Polypeptide signaling molecules may mediate this inductive interaction. Here we report the purification of ARIA, a protein that stimulates the synthesis of muscle acetylcholine receptors, and the isolation of ARIA cDNA. Recombinant ARIA increases acetylcholine receptor synthesis greater than ... More
The accessory subunit of Xenopus laevis mitochondrial DNA polymerase gamma increases processivity of the catalytic subunit of human DNA polymerase gamma and is related to class II aminoacyl-tRNA synthetases.
AuthorsCarrodeguas JA, Kobayashi R, Lim SE, Copeland WC, Bogenhagen DF
JournalMol Cell Biol
PubMed ID10330144
Peptide sequences obtained from the accessory subunit of Xenopus laevis mitochondrial DNA (mtDNA) polymerase gamma (pol gamma) were used to clone the cDNA encoding this protein. Amino-terminal sequencing of the mitochondrial protein indicated the presence of a 44-amino-acid mitochondrial targeting sequence, leaving a predicted mature protein with 419 amino acids ... More
CpABC, a Cryptosporidium parvum ATP-binding cassette protein at the host-parasite boundary in intracellular stages.
AuthorsPerkins ME, Riojas YA, Wu TW, Le Blancq SM
JournalProc Natl Acad Sci U S A
PubMed ID10318953
The intracellular parasite Cryptosporidium parvum develops inside a vacuole at the apex of its epithelial host cell. The developing parasite is separated from the host cell cytoplasm by a zone of attachment that consists of an extensively folded membranous structure known as the feeder organelle. It has been proposed that ... More
Mutations in HYAL1, a member of a tandemly distributed multigene family encoding disparate hyaluronidase activities, cause a newly described lysosomal disorder, mucopolysaccharidosis IX.
AuthorsTriggs-Raine B, Salo TJ, Zhang H, Wicklow BA, Natowicz MR
JournalProc Natl Acad Sci U S A
PubMed ID10339581
Hyaluronan (HA), a large glycosaminoglycan abundant in the extracellular matrix, is important in cell migration during embryonic development, cellular proliferation, and differentiation and has a structural role in connective tissues. The turnover of HA requires endoglycosidic breakdown by lysosomal hyaluronidase, and a congenital deficiency of hyaluronidase has been thought to ... More
Plant ribosome recycling factor homologue is a chloroplastic protein and is bactericidal in escherichia coli carrying temperature-sensitive ribosome recycling factor.
AuthorsRolland N, Janosi L, Block MA, Shuda M, Teyssier E, Miege C, Cheniclet C, Carde JP, Kaji A, Joyard J
JournalProc Natl Acad Sci U S A
PubMed ID10318906
We have isolated a protein, mature RRFHCP, from chloroplasts of spinach (Spinacia oleracea L.) that shows 46% sequence identity and 66% sequence homology with ribosome recycling factor (RRF) of Escherichia coli. RRF recycles ribosomes through disassembly of the posttermination complex. From the cDNA analysis and from the amino-terminal sequencing of ... More
A dominant T cell receptor beta-chain in response to a short ragweed allergen, Amb a 5.
AuthorsHuang SK, Yi M, Palmer E, Marsh DG
JournalJ Immunol
PubMed ID7751655
HLA-DR alpha, beta 1 *1501 (and the closely related DR alpha, beta 1 *1502) heterodimers are the primary class II MHC restriction elements controlling T cell responses to a ragweed pollen allergen, Amb a 5 (M(r) = 5000). Using a novel, quantitative, competitive PCR (QC-PCR) assay and a TCR beta-gene ... More