RapidOut DNA Removal Kit
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RapidOut DNA Removal Kit
Thermo Scientific™

RapidOut DNA Removal Kit

Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations. Complete digestionRead more
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Catalog number K2981
Price (USD)/ Each
112.65
Online exclusive
131.00 
Save 18.35 (14%)
-
Add to cart
Price (USD)/ Each
112.65
Online exclusive
131.00 
Save 18.35 (14%)
Add to cart
RapidOut DNA Removal Kit
Catalog numberK2981
Price (USD)/ Each
112.65
Online exclusive
131.00 
Save 18.35 (14%)
-
Add to cart
Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations. Complete digestion of DNA and safe removal of DNase I from the digestion reaction is ensured without RNA damaging steps, such as heating or organic extraction. First, the RNA sample is treated with recombinant RNase-free DNase I to levels below the limit of detection by routine PCR. DNase I is safely removed subsequently using proprietary DNase Removal Reagent (DRR).

DRR efficiently binds DNase I and the complex is collected at the bottom of the tube by centrifugation. The purified RNA is collected as a supernatant. The RNA after RapidOut procedure is free from DNA contamination and free of DNase I. It is ready to use in different applications including end-point or real-time RT-PCR, cloning, microarrays, and Northern blotting.

Highlights

Efficient—complete ds and ssDNA digestion and proprietary technology for DNase I removal
Rapid—single step sufficient for complete DNase I removal
Safe—no need for toxic organic extractions or RNA-damaging heating steps

Applications

Main Applications
• RNA isolation and RNA analysis, particularly RT-qPCR and RT-PCR (customers performing expression analysis of low transcription level genes.
• Customers performing ds-cDNA synthesis from total RNA preps.

Other applications
• Elimination of DNA from RNA for microinjections and transfection.
• Elimination of DNA from RNA prior microarray analysis.
• Elimination of DNA from RNA prior Northern blot analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
EnzymeDNase
Quantity50 preps
Product TypeRemoval Kit
Unit SizeEach