pcDNA™3.1/V5-His TOPO™ TA Expression Kit

Catalog number:  K480040

 Related applications: Protein Expression

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants.

In addition, the vector includes the following features:

• Strong CMV promoter for high-level, constitutive expression.
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody.
• C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with an Anti-His (C-term) antibody.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: CMV
Key Function: Constitutive Expression
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Delivery Method: Transfection
Cloning Method: TOPO®-TA
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: TOPO-TA Vectors, pcDNA
Constitutive or Inducible System: Constitutive
Product Line: TOPO®, pcDNA™
Product Size: 40 reactions

Contents & storage

2 × Box 1 (store at -20°C)
• 20 µl pcDNA™3.1/V5-His-TOPO® (10 ng/µl)
• 100 µl 10X PCR Buffer
• 10 µl dNTP Mix (50 mM)
• 50 µl Salt Solution
• 1 ml Sterile Water
• 10 µl Control PCR Template (50 ng/µl)
• 10 µl Control PCR Primers (100 ng/µl each)
• 20 µl T7 Sequencing Primer (100 ng/µl)
• 20 µl BGH Reverse Sequencing Primer (100 ng/µl)
• 10 µl Expression Control Plasmid (500 ng/µl)

2 × Box 2 (store at -80°C)
• 21 × 50 µl TOP10 E. coli cells
• 50 µl pUC19 Control DNA (10 pg/µl)
• 6 ml S.O.C. Medium


Manuals & protocols

Recommended products