pcDNA™3.1/V5-His TOPO® TA Expression Kit

Catalog number: K480040

Invitrogen™  Related applications: Protein Expression

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Description

The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants.

In addition, the vector includes the following features:

• Strong CMV promoter for high-level, constitutive expression.
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody.
• C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with an Anti-His (C-term) antibody.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Constitutive Expression
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Delivery Method: Transfection
Cloning Method: TOPO®-TA
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: TOPO-TA Vectors, pcDNA
Constitutive or Inducible System: Constitutive
Product Size: 40 reactions

Contents & storage

2 × Box 1 (store at -20°C)
• 20 µl pcDNA™3.1/V5-His-TOPO® (10 ng/µl)
• 100 µl 10X PCR Buffer
• 10 µl dNTP Mix (50 mM)
• 50 µl Salt Solution
• 1 ml Sterile Water
• 10 µl Control PCR Template (50 ng/µl)
• 10 µl Control PCR Primers (100 ng/µl each)
• 20 µl T7 Sequencing Primer (100 ng/µl)
• 20 µl BGH Reverse Sequencing Primer (100 ng/µl)
• 10 µl Expression Control Plasmid (500 ng/µl)

2 × Box 2 (store at -80°C)
• 21 × 50 µl TOP10 E. coli cells
• 50 µl pUC19 Control DNA (10 pg/µl)
• 6 ml S.O.C. Medium

Documents

Manuals & protocols

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