ViraPower™ Adenoviral Gateway™ Expression Kit

Citas y referencias (7)

Invitrogen™

ViraPower™ Adenoviral Gateway™ Expression Kit

El sistema de expresión adenoviral ViraPower™ de Invitrogen facilita la administración in vitro o in vivo de un gen dianaMás información

Número de catálogo	Cantidad
K493000	1 kit

Número de catálogo K493000

Precio (MXN)

Cantidad:

1 kit

El sistema de expresión adenoviral ViraPower™ de Invitrogen facilita la administración in vitro o in vivo de un gen diana a células de mamíferos que se dividen y no se dividen mediante un adenovirus incompetente en la replicación.El sistema de expresión adenoviral ViraPower™ aprovecha la tecnología de clonación de recombinación Gateway™ para simplificar la clonación, lo que mejora considerablemente la eficacia de la generación de adenovirus recombinantes de alto nivel.Este kit contiene el vector adenoviral adaptado de ⁄CMV⁄V5-DEST Gateway™ que permite la expresión de proteínas de alto nivel del promotor potenciador temprano-inmediato del citomegalovirus humano (CMV), así como la línea celular 293A.

Ventajas clave:
•Produce soluciones madre de gran carga adenovirales de hasta 1 x 1011 pf⁄uml en preparaciones concentradas
• La clonación de recombinación Gateway™ rápida y de alta eficacia evita la recombinación homóloga ineficiente en células humanas o bacterianas
• Administración eficiente del gen de interés para dividir activamente y no dividir células de mamíferos en cultivo o in vivo
• Apto para aplicaciones de alto rendimiento o aplicaciones de transferencia de bibliotecas
• Produce un virus incompetente para la replicación que mejora la bioseguridad del sistema y su uso como vehículo de administración de genes

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Sistema constitutivo o inducibleConstitutivo

Tipo de entregaAdenoviral

Para utilizar con (aplicación)Expresión viral

Tipo de productoKit de expresión adenoviral

Cantidad1 kit

VectorpAd

Método de clonaciónGateway

Línea de productosGateway, ViraPower

PromotorCMV

Etiqueta de proteínaEtiqueta de epítopo V5

Unit SizeEach

Contenido y almacenamiento

Vector pAd⁄CMV⁄V5-DEST Gateway™: almacenar a -20 °C;
Células 293A: almacenar en nitrógeno líquido.
Se garantiza la estabilidad durante 6 meses si se almacena correctamente.

Preguntas frecuentes

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all ViraPower™ Adenoviral Gateway™ Expression Kit FAQs

Citations & References (7)

Citations & References

Abstract

The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit.

Authors:Shimada M, Mahon MJ, Greer PA, Segre GV,

Journal:Endocrinology

PubMed ID:15691895

'We show calcium-dependent, direct binding between the N-terminal portion of the PTH/PTHrP receptor (PTH1R) C-terminal intracellular tail and the calpain small subunit. Binding requires, but may not be limited to, amino acids W474, S475, and W477. The wild-type, full-length rat (r) PTH1R, but not rPTH1R with W474A/W477A substitutions, copurifies with ... More

p116Rip targets myosin phosphatase to the actin cytoskeleton and is essential for RhoA/ROCK-regulated neuritogenesis.

Authors:Mulder J, Ariaens A, van den Boomen D, Moolenaar WH,

Journal:Mol Biol Cell

PubMed ID:15469989

Activation of the RhoA-Rho kinase (ROCK) pathway stimulates actomyosin-driven contractility in many cell systems, largely through ROCK-mediated inhibition of myosin II light chain phosphatase. In neuronal cells, the RhoA-ROCK-actomyosin pathway signals cell rounding, growth cone collapse, and neurite retraction; conversely, inhibition of RhoA/ROCK promotes cell spreading and neurite outgrowth. The ... More

Pulmonary interleukin-23 gene delivery increases local T-cell immunity and controls growth of Mycobacterium tuberculosis in the lungs.

Authors:Happel KI, Lockhart EA, Mason CM, Porretta E, Keoshkerian E, Odden AR, Nelson S, Ramsay AJ,

Journal:Infect Immun

PubMed ID:16113296

Interleukin-23 (IL-23) is a heterodimeric cytokine that shares IL-12 p40 but contains a unique p19 subunit similar to IL-12 p35. Previous studies indicate a greater importance for intact IL-12/23 p40 expression than IL-12 p35 for immunity against Mycobacterium tuberculosis, suggesting a role for IL-23 in host defense. The effects of ... More

Estrogen related receptors stimulate pyruvate dehydrogenase kinase isoform 4 (PDK4) gene expression.

Authors:Zhang Y, Ma K, Sadana P, Chowdhury F, Gaillard S, Wang F, McDonnell DP, Unterman TG, Elam MB, Park EA,

Journal:J Biol Chem

PubMed ID:17079227

The pyruvate dehydrogenase complex (PDC) catalyzes the conversion of pyruvate to acetyl-CoA in mitochondria and is a key regulatory enzyme in the oxidation of glucose to acetyl-CoA. Phosphorylation of PDC by the pyruvate dehydrogenase kinases (PDK2 and PDK4) inhibits PDC activity. Expression of the PDK genes is elevated in diabetes ... More

Cellular distribution, post-translational modification, and tumorigenic potential of human group III secreted phospholipase A2.

Authors:Murakami M, Masuda S, Shimbara S, Ishikawa Y, Ishii T, Kudo I,

Journal:J Biol Chem

PubMed ID:15863501

Human group III secreted phospholipase A(2) (sPLA(2)-III) consists of a central group III sPLA(2) domain flanked by unique N- and C-terminal domains. We found that the sPLA(2) domain alone was sufficient for its catalytic activity and for its prostaglandin E(2) (PGE(2))-generating functions in various cell types. In several if not ... More

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