ViraPower™ Adenoviral Gateway® Expression Kit

Catalog number: K493000

Invitrogen™  Related applications: Antibody & Cell Culture Production | Protein Expression | Viral Vaccine Production

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Description

Invitrogen’s ViraPower™ Adenoviral Expression System facilitates highly efficient, in vitro or in vivo delivery of a target gene to dividing and non-dividing mammalian cells using a replication-incompetent adenovirus. ViraPower™ Adenoviral Expression System takes advantage of the Gateway® Recombination Cloning Technology to simplify cloning, which greatly enhances the efficiency of generating high-titer, recombinant adenovirus. This kit contains the pAd⁄CMV⁄V5-DEST Gateway®-adapted adenoviral vector enabling high level protein expression from human cytomegalovirus (CMV) immediate-early enhancer⁄promoter) as well as the 293A cell line.

Key advantages:
• Produce high titer adenoviral stocks up to 1 x 1011 pfu⁄ml in concentrated preparations
• High efficiency and rapid Gateway® Recombination Cloning bypasses inefficient homologous recombination in human or bacterial cells
• Efficient delivery of the gene of interest to actively dividing and non-dividing mammalian cells in culture or in vivo
• Amenable for high-throughput applications or library transfer applications
• Produces of a replication-incompetent virus that enhances the biosafety of the system and its use as a gene delivery vehicle
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Adenoviral
Cloning Method: Gateway®
Vector Type: pAd
Product Size: 1 kit
Constitutive or Inducible System: Constitutive

Contents & storage

pAd⁄CMV⁄V5-DEST Gateway® Vector: store at -20°C;.
293A Cells: store in liquid nitrogen.
Guaranteed stable for 6 months when properly stored.

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Manuals & protocols

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