Contents & Storage
The BLOCK-iT™ U6 Entry Vector Kit contains two boxes. The cloning box contains four tubes of linearized vector at 5 ng/tube, enough to complete 5 cloning reactions per tube. Also included are 250 μl 10X annealing buffer, 20 μl T4 DNA ligase, 80 μl 5X DNA ligase buffer, 1.4 ng lacZ control oligo, 10 μl lyophilized lacZ control plasmid, 1.5 ml DNase/RNase-free water, and 2 μg each of forward and reverse sequencing primer. Store the vectors, buffers, control oligo and plasmid, water, and sequencing primers at -20°C. The One Shot™ box contains transformation reagents including twenty-one 50-μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and a pUC19 supercoiled control plasmid. Store at -80°C. All reagents are guaranteed stable for 6 months when properly stored.