Flp-In™ Core System

Catalog number: K601002

Invitrogen™  Related applications: Protein Expression

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Our Flp-In™ Core Kit (part of the Flp-In™ Complete System) contains the necessary vectors that allow integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In System involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice. An expression vector containing your gene of interest is then integrated into the genome via Flp recombinase-mediated DNA recombination at the FRT site.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: CMV
Key Function: Targeted Integration
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Zeocin™
Vector Type: pFRT
Product Size: 1 kit
Constitutive or Inducible System: Constitutive

Contents & storage

The Flp-In™ Core Kit includes 20 µg each of pFRT⁄lacZeo, pOG44, pcDNA™5⁄FRT, and pcDNA™5⁄FRT⁄CAT (expression control), and 2 µg each of forward and reverse sequencing primers. Store at -20C.

Documents

Manuals & protocols