Flp-In™ Core System

Catalog number:  K601002

 Related applications:

Protein Expression

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Our Flp-In™ Core Kit (part of the Flp-In™ Complete System) contains the necessary vectors that allow integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In System involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice. An expression vector containing your gene of interest is then integrated into the genome via Flp recombinase-mediated DNA recombination at the FRT site.


Promoter: CMV
Key Function: Targeted Integration
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Zeocin™
Vector Type: pFRT
Constitutive or Inducible System: Constitutive
Product Line: Flp-In™, pcDNA™
Product Size: 1 kit
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.

Contents & storage

The Flp-In™ Core Kit includes 20 µg each of pFRT⁄lacZeo, pOG44, pcDNA™5⁄FRT, and pcDNA™5⁄FRT⁄CAT (expression control), and 2 µg each of forward and reverse sequencing primers. Store at -20C.


Manuals & protocols