pIB/V5-His TOPO® TA Expression Kit

Catalog number: K89020

Invitrogen™  Related applications: Protein Expression

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Description

The pIB/V5-His TOPO® TA Expression Kit offers five-minute cloning of Taq-amplified PCR products directly into the pIB/V5-His-TOPO® expression vector. In addition to being TOPO® Cloning ready, pIB/V5-His-TOPO® includes:

• The OpIE2 promoter for constitutive expression
• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification using nickel-chelating resin
For Research Use Only. Not for use in diagnostic procedures.

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Specifications

Cloning Method: TOPO®-TA
Product Size: 20 reactions
Promoter: OplE2
Protein Tag Position (to your gene): C-terminal
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Vector Type: TOPO-TA Cloning Vectors, pIB

Contents & storage

Each pIB/V5-His TOPO® TA Expression Kit contains two boxes. The pIB/V5-His TOPO® box contains 200 ng of linearized and topoisomerase I-activated pIB/V5-His-TOPO® vector, dNTPs, 10X PCR buffer, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, salt solution, and pIB/V5-His/CAT. Store at -20°C. The One Shot® box contains high-efficiency, single-use, 50 µl aliquots of chemically competent TOP10 E. coli cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. All reagents are guaranteed stable for 6 months when properly stored.

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