IFN gamma ELISA Kit, Human

Catalog number: KAC1231

Novex™  Related applications: Protein Assays and Analysis

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The Human Interferon gamma (IFN gamma, IFNG) ELISA research-use-only kit is to be used for the quantitative determination of IFN gamma in samples (see sample types indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.

Performance characteristics

• Sensitivity: <0.03 IU/mL
• Standard curve range: 1‒30 IU/mL
• Sample type(s): serum, plasma, cell culture supernatant, urine
• Specificity: natural and recombinant human IFN gamma
• Cross-reactivity: see kit manual for cross-species and/or cross-target reactivity
• Sample volume: 50 μL
• Total assay time: 2.25 hours

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Principle of the method

The human IFN gamma solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Target information

IFN-γ (type 2, immune IFN) is structurally and functionally distinct from type 1 (alpha/beta) interferons and acts on a separate receptor. Only one IFN-γ gene has been identified, coding for a 146 AA protein that is post-translationally processed into two glycosylated species of 20 and 25 Kd. Native IFN-γ is pH2-labile, highly basic, and can aggregate to form dimers that are biologically active. IFN-γ is a real lymphokine produced by activated T (and NK) cells. Despite its clear antiviral and cellular growth regulating activities, its immunomodulatory properties are believed to be the most important. IFN-γ is the principal activator of macrophage function (Macrophage Activating Factor, MAF), and it also regulates the pathway of differentiation of myeloid cells. It plays an important role in the growth and differentiation of cytotoxic (and possibly suppressor) T cells, activates NK cells and acts as a B cell maturation factor. It regulates Ig isotype production and inhibits IgE responses. One of the modes of action of IFN-γ is to induce the expression of membrane proteins, such as class 1 and class 2 MHC antigens and adhesion molecules on various cell types, high affinity Fc receptors for IgG on myelomonocytic cells, etc. Integrated in the cytokine network, IFN-γ interacts with other cytokines, in either a synergistic (e.g., TNF) or antagonistic (e.g., IL-4) way.*

* Please refer to ELISA protocol for specific reference citations.

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For Research Use Only. Not for use in diagnostic procedures.


Gene Aliases: IFG, IFI
Accession Number: NP_000610.2, NM_000619.2
Shipping Condition: Wet Ice
Marker: IFN-γ
Gene ID: 3458
Species: Human
Gene Symbol: IFNG
Sensitivity: 0.03 IU⁄mL
Target Gene: interferon, gamma
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 assays
Concentration: 1-30 IU⁄ml
Sample Volume: 50 µl
Protein Family: Cytokines & Receptors
Incubation Time: 2.25 hrs
Detection Method: Colorimetric
Research Category: Immunology
Sample Type (Specific): Plasma, Urine, Serum, Supernatants
For Use With (Equipment): Microplate Reader

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody(s), diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols

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