IL-8 Human ELISA Kit

Catalog number: KHC0082

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Human Interleukin-8 (IL-8, IL8) ELISA research-use-only kit is to be used for the quantitative determination of IL-8 in samples (see sample types indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.

Performance characteristics

• Sensitivity: <5 pg/mL
• Standard curve range: 15.6–1,000 pg/mL
• Sample type(s): serum, plasma, cell culture supernatant
• Specificity: natural and recombinant human IL-8
• Cross-reactivity: see kit manual for cross-species and/or cross-target reactivity
• Sample volume: 50 μL
• Total assay time: 2.5 hours

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Principle of the method

The human IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Target information

IL-8 (also known as NAP-1 for Neutrophil-Activating Peptide) is a chemoattractant protein for neutrophils. This cytokine belongs to a new family of chemotactic peptides called "chemokines". This proinflammatory mediator is secreted by different cells such as monocytes, neutrophils, endothelial cells, fibroblasts after activation, and by mitogen-stimulated T lymphocytes. IL-8 is a key cytokine that has been found in scales of psoriasis patients and in synovial fluid of patients suffering from rheumatoid arthritis or gout. A role for IL-8 in the recruitment of neutrophils in the lung during adult respiratory distress syndrome (ARDS) has been suggested. IL-8 levels in septic shock patients correlates with mortality. In acute graft liver rejection the IL-8 serum levels are markedly increased. The level of IL-8 in these and other conditions may prove to have prognostic significance in these disease conditions.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Symbol: CXCL8
Assay Incubation Time: 2.5 hr
Assay Range: 15.6-1000 pg/mL
Assay Sensitivity: <5 pg/mL
Concentration: 15.6-1000 pg⁄ml
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Gene Aliases: GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1
Gene ID: 3576
Gene Name: C-X-C motif chemokine ligand 8
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Marker: IL-8
Product Line: Novex™
Product Size: 192 assays
Protein Name: Interleukin-8
Sample Type (Specific): Cell Culture Supernatants, Plasma, Serum
Sample Volume: 50 µl
Shipping Condition: Wet Ice
Species: Human
Target (Kit Named): IL-8
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P10145

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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