DNA damage induced by metronidazole in Giardia duodenalis triggers a DNA homologous recombination response.
AuthorsOrdoñez-Quiroz A, Ortega-Pierres MG, Bazán-Tejeda ML, Bermúdez-Cruz RM
JournalExp Parasitol
PubMed ID30237050
'The mechanisms underlying metronidazole (MTZ) resistance in Giardia duodenalis have been associated with decreased activity of the enzymes implicated in its activation including nitroductase-1, thioredoxin reductase and pyruvate-ferredoxin oxidoreductase (PFOR). MTZ activation generates radicals that can form adducts with proteins such as thioredoxin reductase and a- and -ß giardins as ... More
The effect of the melatonin on cryopreserved mouse testicular cells.
AuthorsSaki G, Mirhoseini M, Hemadi M, Khodadadi A, Beygom Talebpour Amiri F
JournalInt J Reprod Biomed
PubMed ID27141545
'After improvements in various cancer treatments, life expectancy has been raised, but success in treatment causes loss of fertility in many of the survived young men. Cryopreservation of immature testicular tissues or cells introduced as the only way to preserve fertility. However, freezing has some harmful effects. Melatonin, a pineal ... More
Inhibition of MAPKinase pathway sensitizes thyroid cancer cells to ABT-737 induced apoptosis.
AuthorsGunda V, Sarosiek KA, Brauner E, Kim YS, Amin S, Zhou Z, Letai A, Parangi S
JournalCancer Lett
PubMed ID28259821
'Bcl2 family proteins play an important role in the resistance of thyroid cancer cells to apoptosis induced by chemotherapeutic drugs and targeted therapies. BH3-profiling of seven fresh primary papillary thyroid cancer (PTC) tumors showed dependence for survival on Bcl-xL (2/7), Bcl2 (2/7), and Mcl-1 (2/7), while the majority of thyroid ... More
Disruption of galectin-3 and galectin-3 binding protein (G3BP) interaction by dietary pectic polysaccharides (DPP) - Arrest of metastasis, inhibition of proliferation and induction of apoptosis.
AuthorsNatarajamurthy SH, Sistla S, Dharmesh SM
JournalInt J Biol Macromol
PubMed ID31356934
'Galectin-3 and galectin-3 binding proteins (G3BP) are implicated as key players in metastasis. In the current study, we evaluated the effect of pectic polysaccharides on galectin-3 and G3BP mediated metastasis in vitro (cells) and in vivo (tissues). In vitro study (double immunostaining) confirms the presence of galectin-3 on the cell ... More
Characterization of the cell-free DNA released by cultured cancer cells.
AuthorsBronkhorst AJ, Wentzel JF, Aucamp J, van Dyk E, du Plessis L, Pretorius PJ
JournalBiochim Biophys Acta
PubMed ID26529550
'The most prominent factor that delays the translation of cell-free DNA (cfDNA) analyses to clinical practice is the lack of knowledge regarding its origin and composition. The elucidation of the former is complicated by the seemingly random fluctuation of quantitative and qualitative characteristics of cfDNA in the blood of healthy ... More
Low-Dose Ionizing ?-Radiation Promotes Proliferation of Human Mesenchymal Stem Cells and Maintains Their Stem Cell Characteristics.
AuthorsCho W, Kim ES, Kang CM, Ji YH, Kim JI, Park SJ, Son Y, Kim CH
JournalTissue Eng Regen Med
PubMed ID30603498
Mesenchymal stem cells (MSCs), which are multipotent and have self-renewal ability, support the regeneration of damaged normal tissue. A number of external stimuli promote migration of MSCs into peripheral blood and support their participation in wound healing. In an attempt to harness the potential beneficial effects of such external stimuli, ... More
FBXO25 regulates MAPK signaling pathway through inhibition of ERK1/2 phosphorylation.
The FBXO25 mediates degradation of ELK-1 and thus inhibits transcriptional activation of immediate early genes (iEG). Here we show that FBXO25 regulates yet another node of this signaling pathway, by decreasing MAPK/ERK activity. We show that induction of FBXO25 reduced ERK1/2 phosphorylation independently of MEK1/2. Accordingly, in HAP1 FBXO25 knockout ... More
Cyclin E and FGF8 are downstream cell growth regulators in distinct tumor suppressor effects of ANXA7 in hormone-resistant cancer cells of breast versus prostate origin.
AuthorsBera A, Leighton XM, Pollard H, Srivastava M
JournalTrends Cancer Res
PubMed ID30369774
Tumor suppressor function of Annexin-A7 (ANXA7) was demonstrated by cancer-prone phenotype in