Catalog number: KIT036

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Assay NGAL (human neutrophil gelatinase-associated lipocalin) levels in human serum, plasma (EDTA, heparin, sodium citrate), tissue extracts, urine, and culture supernatant samples with the BioPorto Diagnostic Human NGAL ELISA Kit.

Features of the Human NGAL ELISA Kit:

Target: natural and recombinant human NGAL (human neutrophil gelatinase-associated lipocalin)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450 nm minus absorbance at 550nm
Assay Range: 4 to 1000 pg/mL
Sensitivity: < 2 pg/mL
Precision: undetermined
Sample Types: cell culture supernatant, tissue extracts, serum, plasma (EDTA, sodium citrate and heparin) and urine
Total Assay Time: 3.5 hours
Sample Size: 100 µL of 10-fold diluted sample per well
Specificity: The two monoclonal antibodies against human NGAL used in the assay have been shown to bind to different preparations of recombinant human NGAL and to give a single band at 25kDa on Western blot analysis of a reduced postnuclear supernatant from human neutrophils.

This human NGAL ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of neutrophil gelatinase-associated lipocalin (NGAL). The assay is a sandwich ELISA performed in microplate wells coated with a monoclonal antibody against human NGAL. Bound NGAL is detected with another monoclonal antibody labeled with biotin and the assay is developed with horseradish peroxidase (HRP)-conjugated streptavidin and a color-forming substrate. The assay is a four-step procedure.

NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are typically small secreted proteins characterized by their ability to bind small, hydrophobic molecules in a structurally conserved pocket formed by beta-pleated sheet, to bind to specific cell-surface receptors and to form macromolecular complexes. NGAL has many synonyms: it also known as NL (neutrophil lipocalin; HNL: human NL), lipocalin 2, oncogene protein 24p3 or uterocalin (in the mouse) and neu-related lipocalin or 25kDa alpha-2-microglobulin-related protein (in the rat). Human NGAL consists of a single disulfidebridged polypeptide chain of 178 amino-acid residues with a calculated molecular mass of 22kDa, but glycosylation increases its apparent molecular mass to 25 kDa. In neutrophils (neutrophilic polymorphonuclear leukocytes) and urine it occurs as monomer, with a small percentage of dimer and trimer, and also in complex with 92-kDa human neutrophil type IV collagenase, also called gelatinase B or matrix metalloproteinase-9 (MMP-9). NGAL is often used as a marker of acute renal injury which may lead to acute renal failure.
For Research Use Only. Not for use in diagnostic procedures.


Species: Human
Product Size: 96 assays
Protein Family: Growth Factors & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols