Histogene™ LCM Frozen Section Staining Kit, includes dehydration chemicals, stain, and slides - FAQs

I am using the Histogene LCM Frozen Section Staining Kit and the targeted cells do not lift from the slide during LCM. What is the problem?

Here are possible causes and solutions:

1. The sample may contain residual water. Ensure that the ethanol solution is fresh. Ethanol is hygroscopic. Keep the ethanol bottle tightly capped, and do not pour ethanol solution until you are ready to use it. If you suspect that the 100% ethanol solution has absorbed water, purchase a new bottle.

2. The sample may have dried between protocol steps. Carry out the “Staining and Dehydration” segment of the protocol at a steady pace.