Invitrogen™

LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Catalog number: L3224
Invitrogen™

LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Catalog number: L3224
Catalog Number
L3224
also known as L-3224
Unit Size
1 kit
Price (USD)
576.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
L3224
also known as L-3224
1 kit
576.00
Your Price:
***
Product Overview
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The LIVE/DEAD™ Viability/Cytotoxicity Kit is a quick and easy two-color assay to determine viability of cells in a population based on plasma membrane integrity and esterase activity. The kit can be used in flow cytometry, fluorescence microscopy, and with fluorescence microplate readers.

Advantages Over Alternative Methods Include:


•  Faster
•  Safer
•  More sensitive
•  Less expensive

Easily Use with a Wide Variety of Techniques and Cell Types
Ubiquitous intracellular esterase activity and an intact plasma membrane are distinguishing characteristics of live cells. The LIVE/DEAD™ Viability/Cytotoxicity Kit quickly discriminates live from dead cells by simultaneously staining with green-fluorescent calcein-AM to indicate intracellular esterase activity and red-fluorescent ethidium homodimer-1 to indicate loss of plasma membrane integrity. It is adaptable to most eukaryotic cells where cytotoxic conditions produce these cellular effects. The assay is useful with a variety of fluorescence-detection methodologies.

Sensitive, Safe, and Efficient

The LIVE/DEAD™ Viability/Cytotoxicity Kit is more sensitive than Trypan blue exclusion, a commonly used method for live/dead cell discrimination. The cost-effective LIVE/DEAD™ Viability/Cytotoxicity Kit is highly sensitive due to the bright fluorescence of both dyes upon interacting with either live (for calcein-AM) or dead (for ethidium homodimer-1) cells. Background levels are low due to the fact that both dyes are virtually non-fluorescent prior to interacting with cells.

LIVE/DEAD™ Assays Available for a Broad Range of Applications
A selection of Invitrogen LIVE/DEAD™ Viability Assays is offered for mammalian cells, bacteria, yeast and fungi, as well as Fixable Dead Cell Stain Kits for use in intracellular staining for flow cytometry. All LIVE/DEAD™ assays provide quick, positive discrimination between viable and non-viable cells.

Related Link
•  Learn More and See the Full Range of LIVE/DEAD™ Assay Products.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Mammalian Cells
Detection Method
Fluorescent
For Use With (Application)
Viability Assay
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product Type
Viability/Cytotoxicity Kit
Dye Type
Other Label(s) or Dye(s)
Format
Tube(s), 96-well plate, Slide(s)
Product Line
LIVE/DEAD™
Shipping Condition
Room Temperature

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

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