Kit de citotoxicidad/viabilidad LIVE/DEAD™, para células de mamíferos

Citas y referencias (210)

Invitrogen™

Kit de citotoxicidad/viabilidad LIVE/DEAD™, para células de mamíferos

El kit de citotoxicidad/viabilidad LIVE/DEAD® es un ensayo de dos colores rápido y sencillo para determinar la viabilidad de lasMás información

Número de catálogo	Cantidad
L3224	1 kit

Número de catálogo L3224

Precio (MXN)

Cantidad:

1 kit

El kit de citotoxicidad/viabilidad LIVE/DEAD® es un ensayo de dos colores rápido y sencillo para determinar la viabilidad de las células en una población basada en la actividad de la esterasa y la integridad de la membrana plasmática. El kit puede utilizarse en citometrías de flujo, microscopía de fluorescencia y lectores de microplacas de fluorescencia.

Las ventajas en comparación con otros métodos incluyen:

• Más rápido
• Más seguro
• Más sensible
• Más barato

Fácil de usar con una amplia variedad de técnicas y tipos celulares
La actividad de la esterasa intracelular ubicua y una membrana plasmática intacta son características distintivas de las células vivas. El kit de citotoxicidad/viabilidad LIVE/DEAD® discrimina rápidamente las células vivas de las muertas mediante la tinción simultánea con calceína AM verde fluorescente para indicar actividad de esterasa intracelular y homodímero-1 de etidio rojo fluorescente para indicar la pérdida de integridad de la membrana plasmática. Se puede adaptar a la mayoría de las células eucarióticas en las que las condiciones citotóxicas producen estos efectos celulares. El ensayo es útil con una variedad de metodologías de detección de fluorescencia.

Sensible, seguro y eficiente
El kit de viabilidad/citotoxicidad LIVE/DEAD® es más sensible que la exclusión azul de tripano, un método comúnmente utilizado para la discriminación de células vivas/muertas. El kit de citotoxicidad/viabilidad LIVE/DEAD® es rentable y muy sensible debido a la brillante fluorescencia de ambos colorantes al interactuar con las células vivas (calceína AM) o muertas (homodímero-1 de etidio). Los niveles de fondo son bajos debido al hecho de que ambos colorantes son prácticamente no fluorescentes antes de interactuar con las células.

Hay ensayos LIVE/DEAD® disponibles para una amplia gama de aplicaciones
Se ofrece una selección de ensayos de viabilidad Invitrogen LIVE/DEAD® para células de mamíferos, bacterias, levaduras y hongos, así como kits de tinción de células muertas fijables para su uso en tinción intracelular para citometría de flujo. Todos los ensayos LIVE/DEAD® proporcionan una discriminación rápida y positiva entre células viables y no viables.

Enlace relacionado
• Obtener más información y ver la gama completa de productos de ensayo LIVE/DEAD®.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de célulaCélulas de mamíferos

DescripciónKit de viabilidad/citotoxicidad LIVE/DEAD™, para células de mamíferos

Método de detecciónFluorescente

Tipo de coloranteOtras etiquetas o colorantes

FormatoTubos, placa de 96 pocillos, portaobjetos

Cantidad1 kit

Condiciones de envíoTemperatura ambiente

ColorVerde, rojo

Emission494, 528 nm

Excitation Wavelength Range517, 617 nm

Para utilizar con (aplicación)Ensayo de viabilidad

Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo, Lector de microplacas

Línea de productosLIVE/DEAD

Tipo de productoKit de viabilidad/citotoxicidad

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

I need to use a dead cell control for my viability assay. Do you have a protocol for killing cells for this?

Heat killing is commonly used. Place your cells in a tube in buffer and heat at 60^oC for 20 minutes. You can also kill your cells by fixing them with ice cold 70% ethanol for 15 minutes. The ethanol-killed cells can then be stored at -20^oC until needed, at which point you wash out the ethanol and replace with buffer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?

This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store the LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells (Cat. No. L3224)?

We recommend storing the LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells (Cat. No. L3224) in the freezer at -5 degrees C to -30 degrees C and protected from light.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What fluorescent viability assays can I use on the Countess II FL automated cell counter?

We have validated the following kits for use on the Countess II FL Automated Cell Counter:

LIVE/DEAD Viabilty/Cytoxicity Kit (Cat. No. L3224) containing calcein AM and ethidium homodimer-1
ReadyProbes Cell Viability Imaging Kit, Blue/Green (Cat. No. R37609)
ReadyProbes Cell Viability Imaging Kit, Blue/Red (Cat. No. R37610) containing NucBlue Live/NucGreen Dead and NucBlue Live/propidium iodide
See this Application Note for details - https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/Laboratory%20Instruments/Files/0415/CO014723-Countess-II-FL-Viability-Appnote_FHR.pdf.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (210)

Citations & References

Abstract

Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.

Authors:Jackson WF,Huebner JM,Rusch NJ

Journal:Microcirculation (New York, N.Y. : 1994)

PubMed ID:9110282

OBJECTIVE: The goal of the present study was to develop a method to isolate viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain and ... More

Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.

Authors:Jackson WF,Huebner JM,Rusch NJ

Journal:Microcirculation (New York, N.Y. : 1994)

PubMed ID:8930888

OBJECTIVE: The goal of the present study was to develop a method to isolate enzymatically viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain ... More

Calcein: a novel marker for lymphocytes which enter lymph nodes.

Authors:Weston SA, Parish CR

Journal:Cytometry

PubMed ID:1451604

Previous studies have identified unique cell surface antigens which are associated with the specific binding of lymphocytes to high endothelial venules (HEV). Evidence is presented in this paper which demonstrates that uptake of the fluorescent dye calcein by lymphocytes represents an additional marker for the lymph node homing subpopulation of ... More

Successful storage of peripheral nerve before transplantation using green tea polyphenol: an experimental study in rats.

Authors:Ikeguchi R, Kakinoki R, Okamoto T, Matsumoto T, Hyon SH, Nakamura T

Journal:Exp Neurol

PubMed ID:14769360

Green tea polyphenol is known to act as a buffer, reducing biological responses to oxidative stress. Several effects of polyphenol have been reported, such as protection of tissue from ischemia, antineoplasmic and anti-inflammatory effects, and suppression of arteriosclerosis. In this study, we investigated whether peripheral nerve segments could be kept ... More

Human stem cell delivery for treatment of large segmental bone defects.

Authors:Dupont KM, Sharma K, Stevens HY, Boerckel JD, García AJ, Guldberg RE,

Journal:Proc Natl Acad Sci U S A

PubMed ID:20133731

'Local or systemic stem cell delivery has the potential to promote repair of a variety of damaged or degenerated tissues. Although various stem cell sources have been investigated for bone repair, few comparative reports exist, and cellular distribution and viability postimplantation remain key issues. In this study, we quantified the ... More

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