Citas y referencias (12)

Invitrogen™

LIVE/DEAD™ Yeast Viability Kit

El kit de viabilidad de levaduras LIVE/DEAD® combina una novedosa sonda fluorescente de dos colores para la viabilidad de levaduras,Más información

Número de catálogo	Cantidad
L7009	1 kit

Número de catálogo L7009

Precio (MXN)

Cantidad:

1 kit

El kit de viabilidad de levaduras LIVE/DEAD® combina una novedosa sonda fluorescente de dos colores para la viabilidad de levaduras, FUN® 1, con un reactivo fluorescente de etiquetado de superficies fúngicas, Calcofluor® White M2R. La integridad de la membrana plasmática y la función metabólica de los hongos son necesarias para convertir la tinción intracelular amarillo-verde fluorescente de FUN® 1 en estructuras intravacuolares rojo-anaranjadas. Calcofluor White M2R etiqueta la quitina de la pared celular con fluorescencia azul independientemente del estado metabólico.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de célulaCélulas de levadura

DescripciónKit de viabilidad de levaduras LIVE/DEAD™

Método de detecciónFluorescente

Tipo de coloranteOtras etiquetas o colorantes

Longitud de onda de emisión530, 620, 500, 550 nm

Longitud de onda de excitación485, 450 nm

FormatoTubos, placa de 96 pocillos, portaobjetos

Cantidad1 kit

Condiciones de envíoTemperatura ambiente

ColorAmarillo-verde, rojo-naranja, azul

Para utilizar con (aplicación)Ensayo de viabilidad

Para utilizar con (equipo)Microscopio de fluorescencia, Fluorímetro, Citómetro de flujo, Lector de microplacas

Línea de productosLIVE/DEAD

Tipo de productoKit de viabilidad de levadura

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

Which species of yeast/fungi can I use with the LIVE/DEAD Yeast Viability Kit?

The LIVE/DEAD Yeast Viability Kit has been tested on the following fungi: S. cerevisiae (five different strains), Candida pseudotropicalis, Neurospora crassa and Aspergillus nidulans. A good correlation between the results obtained with the LIVE/DEAD Yeast Viability Kit and those obtained with standard plate counts was achieved with both S. cerevisiae and C. pseudotropicalis. Tests have been performed on both logarithmically growing cultures and cells that have undergone different forms of environmental stress

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can FUN 1 stained cells be examined by flow cytometry?

Yes. Use a 488 nm laser line and standard FITC and PE channels for two-color detection of green (dead/metabolically inactive cells) and red (live, metabolically active cells) emission.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Live, metabolically active fungi transport FUN 1 into vacuoles to give a red-shifted fluorescence versus green/yellow fluorescence in the nucleus and cytoplasm of dead or metabolically-inactive cells. Is this a reliable indicator of fungal viability?

No. FUN 1 accumulates into vacuoles by an unknown transport pathway, but any mutants/ recombinant cells or experimental treatments that result in a deficiency or block in vesicle-mediate transport into vacuoles may result in cells that do not have red vacuoles, even though the cells are live and metabolically active. For more information see J Microbiol Methods 78:208 (2009).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (12)

Citations & References

Abstract

In vitro growth and analysis of Candida biofilms.

Authors:Chandra J, Mukherjee PK, Ghannoum MA,

Journal:Nat Protoc

PubMed ID:19180075

'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion ... More

Mechanistic aspects in the generation of apparent ultrahigh efficiencies for colloidal (microbial) electrokinetic separations.

Authors:Armstrong DW, Girod M, He L, Rodriguez MA, Wei W, Zheng J, Yeung ES

Journal:Anal Chem

PubMed ID:12433083

'Under specific experimental conditions, the electrokinetic separation of certain microorganisms can produce peaks of very high apparent efficiencies (approximately 10(6)-10(10) theoretical plates/m). This is unusual in that no deliberate focusing mechanism was employed. To investigate this process further, the separation was monitored in real time using a charge-coupled device (CCD) ... More

Pleiotropic effects of HIV-1 protein R (Vpr) on morphogenesis and cell survival in fission yeast and antagonism by pentoxifylline.

Authors:Zhao Y, Yu M, Chen M, Elder RT, Yamamoto A, Cao J

Journal:Virology

PubMed ID:9657945

'Expression of HIV-1 Vpr causes cell cycle G2 arrest, change in cell shape, and cell death over a large evolutionary distance ranging from human to yeast cells. As a step toward understanding these highly conserved Vpr functions, we have examined the effect of Vpr on cytoskeletal elements and the viability ... More

Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of efflux pumps and membrane sterols.

Authors:Mukherjee PK, Chandra J, Kuhn DM, Ghannoum MA

Journal:Infect Immun

PubMed ID:12874310

'Candida albicans biofilms are formed through three distinct developmental phases and are associated with high fluconazole (FLU) resistance. In the present study, we used a set of isogenic Candida strains lacking one or more of the drug efflux pumps Cdr1p, Cdr2p, and Mdr1p to determine their role in FLU resistance ... More

Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells.

Authors:Henry-Stanley MJ, Garni RM, Wells CL,

Journal:J Microbiol Methods

PubMed ID:15369865

'The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and ... More

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