Novex™ IEF Anode Buffer (50X) - FAQs

Novex™ IEF Anode Buffer (50X) - FAQs

View additional product information for Novex™ IEF Anode Buffer (50X) - FAQs (LC5300)

3 product FAQs found

When staining IEF gels with the Colloidal Blue Staining Kit, is it necessary to use the fixing solution?

Yes, the fix serves two purposes: it fixes the sample in the IEF gel and it helps to remove gel background.

If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.

High background is caused by ampholytes remaining in the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the components of the ZOOM IEF Fractionator?

The ZOOM IEF Fractionator includes the following components:

Chamber Assembly Tube with Anode Reservoir
Spill Trough with Cathode Reservoir Lid
Sample Chambers (7)
Sample Chamber O-ring Seals, red (10)
Sample Chamber Caps with O-rings (7)
Cathode End Sealer
Anode End Sealer
Cathode End Screw Cap
Spacers, black (8)
Spares Box 1
Sample Chamber O-ring Seals (8)
Sample Chamber Caps with O-rings (7)
Spares Box 2
Cathode Chamber Seals (2)
Spacers, black (8)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the storage conditions for the ZOOM Disks, ZOOM Carrier Ampholytes, ZOOM Focusing buffers, ZOOM Cathode buffer, IEF Anode buffer, and IEF Cathode buffer?

We recommend storing the ZOOM Disks, ZOOM Carrier Ampholytes, ZOOM Focusing buffers, ZOOM Cathode buffer, IEF Anode buffer, and IEF Cathode buffer at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.