Invitrogen™

MagicMark™ XP Western Protein Standard

Catalog number:  LC5603

Related applications:

Protein Electrophoresis & Western Blotting

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Description

MagicMark XP Western Protein Standard consists of nine recombinant proteins (20–220 kDa), each of which contains an IgG binding site. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot. The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent). The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).
• Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using western blot detection method

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: User defined detection system
Gel Compatibility: Novex® Tricine Gels, Novex® Tris-Glycine Gels, NuPAGE® Bis-Tris Gels, NuPAGE® Tris-Acetate Gels, SDS-PAGE Gels
Number of Markers: 9
Product Line: MagicMark™
Ready to Load: Yes
Shipping Condition: Dry Ice
Size Range: 20 to 220 kDa
System Type: Western Blotting, SDS-PAGE
Kit Contents: 50μL of ready-to-use standardmixture is provided in a plastic vial; Loading buffer consists of 125mM Tris-HCl (pH 6.8), 10mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue
Molecular Weight: 220, 120, 100, 80, 60, 50, 40, 30, 20 kDa
Stain Type: Unstained
Quantity: 50 μL

Contents & storage

50 µl of ready-to-use standard mixture is provided in a plastic vial. Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue. Store at -20°C.