Invitrogen™

BenchMark™ His-tagged Protein Standard

Catalog number:  LC5606

 Related applications:

Protein Electrophoresis & Western Blotting

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Description

BenchMark His-tagged Protein Standard is designed as a positive control and for molecular weight sizing in his-tagged fusion protein detection. Each of the ten standard proteins (10-160 kDa) contains a 6X His-tag, enabling the detection of standard proteins with the InVision™ His-tag In-gel Stain (Cat. no. LC6030) or with Anti-His(C-term) Antibody (Cat. no. R930-25) using western detection. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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Applications
• Verification of His-tagged fusion protein detection using InVision In-Gel Stain or an Anti-His(C-term) antibody
• Sizing of proteins on SDS-polyacrylamide gels and western blots

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: InVision™ His-tag In-gel Stain
Molecular Weight: 160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDa
Number of Markers: 10
Gel Compatibility: Novex® Tricine Gels, Novex® Tris-Glycine Gels, NuPAGE® Bis-Tris Gels, SDS-PAGE Gels
Product Line: BenchMark™
Product Size: 125 µL
Size Range: 10 to 160 kDa
Stain Type: Unstained
System: SDS-PAGE
Shipping Condition: Dry Ice

Contents & storage

125 µl (25 applications of 5 µl each) is provided in loading buffer consisting of Tris-HCl, SDS, Glycerol, DTT, and Coomassie® Blue G-250. Store at -20°C. Avoid repeated freezing and thawing.

Documents

Manuals & protocols