HiMark Unstained High Molecular Weight (HMW) Protein Standard contains nine proteins in the range of 40–500 kDa designed for analysis of high molecular weight proteins. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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To obtain the best results and separation of high molecular weight proteins, we recommended using Tris-Acetate gels. HiMark Pre-Stained High Molecular Weight Protein Standard can also be used with NuPAGE 4–12% Bis-Tris gels and NuPAGE MOPS Running Buffer and with Novex 4% Tris-Glycine gels.

Applications
• Accurate protein sizing of large molecular weight proteins on SDS-polyacrylamide gels and western blots