HiMark Pre-Stained Protein Standard is designed for analysis of high molecular weight proteins and consists of nine protein bands ranging from 30–460 kDa. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

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To obtain the best results and separation of high molecular weight proteins we recommended using Tris-Acetate gels. HiMark Pre-Stained High Molecular Weight Protein Standard can also be used with NuPAGE 4–12% Bis-Tris gels and NuPAGE MOPS Running Buffer and with Novex 4% Tris-Glycine gels.

Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-PAGE gels and western blots